![]() annuum and provide critical resources for the genetic improvement of pepper production. chinense exhibit a relatively closer interspecific relationship with C. annuum is most widely cultivated for use as food, spice, ornaments, and medicine around the world. ![]() Introduction Capsicum is a member of the Solanaceae family and consists of the following five most important cultivated species, C. ![]() Competing Interests: The authors have declared that no competing interests exist. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. PLOS ONE | DOI:10.1371/journal.pone.0119389 Ma1 / 15 Map Construction and Mapping QTLs Affecting Flowering Time China (No. The InDel/SSR-based interspecific genetic map, QTLs and candidate genes obtained by the present study will be useful for the downstream isolation of flowering timerelated gene and other genetic applications for pepper. With the accomplishment of pepper whole genome sequencing and annotations (release 2.0), 153 candidate genes were predicted to embed in the Nle2.2 region, of which 12 important flowering related genes were obtained. The major QTL on P2 was confirmed based on another subset of the same F2 population (n = 147) in 2014 with selective genotyping of markers from the BY map. Based on the BY map, one major and five minor QTLs affecting the number of leaves on the primary axis (Nle) were detected on chromosomes P2, P7, P10 and P11 in 2012. Comparative analysis of the genetic and physical map based on the anchored markers showed that the BY map covered nearly the whole pepper genome. The BY map consisted of 13 linkage groups (LGs) and spanned a total genetic distance of 1,249.77 cM with an average marker distance of 5.60 cM. Of these, a total of 224 simple PCR-based markers, including 129 InDels and 95 SSRs, were validated and integrated into a map, which was designated as the BY map. To accelerate the development and application of molecular markers and identify the QTLs affecting the flowering time-related trait in pepper, a total of 1,038 pairs of InDel and 674 SSR primers from different sources were used for genetic mapping using the F2 population (n = 154) derived from a cross between BA3 (C. * Abstract Re-sequencing permits the mining of genome-wide variations on a large scale and provides excellent resources for the research community. 201201) and the Guizhou Province and Zunyi City Science and Technology Cooperation Project of 1 College of Horticulture, South China Agricultural University, Guangzhou, China, 2 Pepper Institute, Zunyi Academy of Agricultural Sciences, Zunyi, Guizhou, China, 3 Maize Research Institute of Sichuan Agricultural University / Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Chengdu, China, 4 College of Agriculture, Guangxi University, Nanning, China, 5 College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou, China ☯ These authors contributed equally to this work. Funding: This work was supported by the Guangdong Natural Science Foundation of China (S2011030001410), the National High Technology Research and Development Program (“863” Program) of China (2012AA100103), the National Natural Science Foundation of China (31372076), the Zunyi City Natural Science Foundation of China (No. Data Availability Statement: All relevant data are within the paper and its Supporting Information files. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Nelson, The University of Western Australia, AUSTRALIA Received: NovemAccepted: JanuPublished: MaCopyright: © 2015 Tan et al. (2015) Construction of an Interspecific Genetic Map Based on InDel and SSR for Mapping the QTLs Affecting the Initiation of Flower Primordia in Pepper (Capsicum spp.). ![]() RESEARCH ARTICLE Construction of an Interspecific Genetic Map Based on InDel and SSR for Mapping the QTLs Affecting the Initiation of Flower Primordia in Pepper (Capsicum spp.) Shu Tan1☯, Jiao-Wen Cheng1☯, Li Zhang1☯, Cheng Qin1,2,3, Ding-Guo Nong4, Wei-Peng Li1, Xin Tang1, Zhi-Ming Wu5, Kai-Lin Hu1* a11111 OPEN ACCESS Citation: Tan S, Cheng J-W, Zhang L, Qin C, Nong D-G, Li W-P, et al.
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![]() Unlike mounting a remote volume, Cloud Sync works perfectly offline. The services keep it all straight and log each device and transaction. Each Cloud Sync client can sync with many different Cloud Stations and each Cloud Station allows multiple clients. The Synology design allows for a many-to-many configuration. ![]() As with the Synology Cloud Station app on the NAS, the Mac app has optional sync profiles to restrict file types. The menu bar provides quick access to the local sync directory as well as the settings. Individual folders can be disabled for syncing to the Synology NAS and all other connected devices.įollowing the Dropbox design, which is fine with me, Cloud Sync runs as a menu bar application to indicate current syncing status and lists recent file changes. There’s also selective syncing for subfolders. Synology share links use a short (another Synology service) URL but these simply redirect to your own NAS through the magic of Synology DDNS and do not reside on a hosted service. Right click to access file versions or to create a Synology sharing link. Subtle overlays are displayed on the file icons to indicate their current sync status.Ĭloud Station also adds a new contextual menu for files to OS X. 3Īdding, deleting, or changing files on the Mac causes them to sync to the Synology. From there, it behaves much like Dropbox. Once installed, it’s a simple matter of giving it a Cloud Station ID and telling it which local directory to use for syncing on the Mac. The client application is downloaded directly from the Synology NAS interface or from the Synology download page. The NAS to NAS syncing is nice but it’s the Mac syncing client that starts to show off the real power behind Cloud Station. ![]() There were no issues with “normal” sized syncs are syncing multi Gigabyte files.Īll of this is available through the Synology web interface, which means I can add or delete files from almost any computer with an Internet connection and changes sync everywhere. The syncing service would eventually die or hang and require a restart of the NAS to fix it. The biggest issue I encountered was with syncing very large collections of files (tens of thousands). In my testing, the reliability and performance is fairly stable and mostly dependent on how reliable the two NAS Internet connections are. Once configured, every file added, deleted or changed on either end of the Cloud Station is automatically synced between the two NAS devices. Setup is pretty easy but managing the permissions can be a bit confusing if you have no experience with these options. Other Synology owners can sync through my Cloud Station using the companion Cloud Sync service on their own NAS. Versions can be restored or downloaded as a new file. This is a basic function of the Synology OS and it’s extended to the Cloud Station applications on other devices. Similar to Dropbox Pro, Cloud Station provides optional versioning of the synced files. If you don’t want your friend’s collection of animated GIFs, then setup a profile to exclude syncing files with the. And this can all be segregated based on permissions so that private content stays private.Ĭloud Station provides optional sync profiles for configuring what type of content can be kept in sync. Everything they add to their shared folder will automatically be synced between NAS devices. Why would this be useful? Because you can share a Synology Cloud Station with another Synology user. You can setup access through Synology permissions so that different users have different access. ![]() In some ways, it’s more powerful than Dropbox. You point it at some directories on the Synology and it looks for changes and notifies clients when they need to get a new file. It’s configured with a Synology “QuickConnect ID” (which uses Synology’s free DDNS service) to make connecting other devices to the service easy. The Cloud Station service runs on the Synology as a free user installed application. Both are fast for me, but whatt I really like is that the files are only ever on devices under my physical control. 1 The sync application for the Mac works well and the DS Cloud iOS app is very functional. The announcement of a new Amazon unlimited storage option is compelling but the lack of options for access as well as limited app support will likely keep me using Dropbox.īut, I find myself using my own hosted Cloud Station on my Synology NAS more often as my way of getting files moved between devices. I’ve been a dedicated Dropbox user for many years and continue to pay for their “Pro” consumer product. BitTorrent Sync, Dropbox, Amazon Cloud, Google Drive - There’s no shortage of ways I can store files on the Internet. Then the learner is given a sentence with several options for a word or phrase, and the student chooses the correct option. ![]() In grammar lessons, the program firstly shows the learner several examples of a grammatical concept, and in some levels the word or words the learner should focus on are highlighted. Grammar lessons cover grammatical tense and grammatical mood. 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Vent window posts are typically in bad shape and I’ve seen a few cases where nothing is done to make them look more appealing. Vent windows, door window and quarter window trim all get polished to a high luster finish, then the roof rail, windshield and back window moldings get polished.ĭoor and vent window glass should be protected with duct tape along the trim as a precautionary measure. ![]() This process takes a lot of hard work and dedication but the results are well worth it, even on new parts. If you relax for just a moment the buffing wheel will take the piece you are working on and throw it across the room or wrap it around the wheel. Polishing the stainless steel is the most labor intensive task among the three, takes the most time, requires a lot of patients and your full attention if using a high speed buffer. The front windshield and back window both need to be replaced so I’ll be ordering new or try to locate some nice used pieces. Most of my glass had water spots so I used 3M Perfect-It rubbing compound and super fine steel wool to remove them this is a lot of work so if you know an easier way then please leave a comment. I then inspect the glass, stainless trim and frame to make sure its usable. I don’t want to put out the expense or labor to replace the glass so I’ll clean and inspect all of the glass to determine the usable condition of each piece.Įach piece of glass is cleaned with lacquer thinner and then washed thoroughly with soap and water. If I had clear glass I could purchase new assemblies that come complete. Purchasing new Mustang glass can be expensive, and in my case if I buy new tinted glass I would have to reuse the existing stainless trim and window frames. To remove the paint I used some lacquer thinner to scrub it off, then I continued with the thinner and a tooth brush cleaning the remaining paint and old grease from the assemblies.Īfter an initial cleaning the door latch assemblies and the door strikers got a low pressure run through the bead blast cabinet, then received a a dusting of protective paint. My door latches and door strikers like so many other had been painted over but they are in really good shape and worthy of restoration. If I decide to go new I will have a restored set that I can make available to somebody through or Door Latch RestorationĪlthough I could purchase new door latches and strikers I am going to spend a little time restoring these, and then I’ll make that decision. The window guides and tracks, brackets and bolts all get cleaned thoroughly, bead blasted and painted when required. The major mechanical parts like the window regulators, door latches and strikers will be replaced with new reproduction or good used parts if they are broken or beyond simple restoration. The work to be performed consists of cleaning, bead blasting and painting. This task will deal with the internal and mechanical parts of the doors, quarter and vent windows. Mechanical and Internal Parts Restoration ![]() Stainless Steel Moldings (clean and polish).Mechanical and internal (clean, blast, paint).I’ve had my door and window broken out into project groups so I can manage my parts purchasing and restoration requirements.įor organizing and ordering parts this is great but this is not how I want the restoration part of the task grouped so I’ve decided to group things by the type of work to be performed. The vent window and quarter window frames will go out to the chrome shop, they have been disassembled with the glass and trim removed. 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RELATED: Parasite Eve, Square's Answer to Resident Evil, Deserves a PS5 Remake Parasite Eve is a 1998 action role-playing survival horror video game developed and published by Square. ![]() In severe attacks, all voluntary muscles except the diaphragm may be affected.
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![]() The free version, Relax Lite, is designed for use while sitting in a chair and runs for 15 minutes. Johnson speaks slowly and softly, prompting you to relax one muscle at a time. Relax Lite with Andrew Johnson: If you have trouble unwinding at the end of the day, try one of clinical hypnotherapist Andrew Johnson's guided relaxation apps. There's even a thunderstorm mode that is both entertaining and invigorating.ĥ. You can also feed the fish and "touch" the water. The free version of the Pocket Pond app allows you to add additional fish, lilypads and dragonflies to the pond. Pocket Pond: When you have too much on your mind, take a break and watch colorful fish swim through crystal clear water while soft nature sounds play in the background. The app comes with four soothing background sounds or you can add songs from your iTunes playlist for a more personalized experience.Īvailable on: iPad and Android (Zen Garden Lite)Ĥ. Free your mind as you rake patterns in the sand and decorate with realistic stones and leaves. Zen Spaces: If you're faced with a creative block, try spending a few minutes rearranging your own virtual Zen garden. Related: 7 Simple Ways to Relieve Stress Nowģ. You can also use the mixer to combine sounds for your own personal soundtrack or the night timer to automatically fade the sounds as you fall asleep. The Relaxing Sounds Of Nature Lite app comes with nature sounds such as whale calls, rain and a variety of beach sounds. Relaxing Sounds Of Nature Lite: If your work area is filled with distractions, put on headphones and launch one of the soothing sounds in this app. Relax Now is a free meditation app that’s based on hypnosis meditation. The app allows you to adjust the inhale and exhale length, as well as the number of cycles.Ģ. The app is available for iOS and Android users and it’s free to download but keep in mind that it comes with optional in-app purchases. 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The employee login is used to sign in to Studiometry each time you launch the app. This can be set or changed in Studiometry's Preferences Window > Employees section, or via the Studiometry Cloud web site. The Admin Login is completely separate from your Studiometry data, and is not used to log in to any employee or account within Studiometry itself.Įvery employee on your Studiometry Cloud account will have their own employee login consisting of a username and a password. Uploading a new database or replacing the existing database on your Studiometry Cloud account.Logging in to manage your account on the Studiometry Cloud web site.The email on file for the admin login is where all Studiometry Cloud account notifications will be sent. Admin LoginĮach Studiometry Cloud account will have one admin login consisting of an email and a password. 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Stunning: Amanda took to her Instagram on Friday to share an array of gorgeous photographs of herself out for a luxurious evening out in the French capitalĪnd she put on a very racy display in one final snap, which saw her putting her incredible figure on full display as she lay across a table in her skimpy ensemble.Īlongside the picture, she cheekily penned: 'Making Whoooopie.' In another picture, Amanda gave an insight into her lavish evening out as she showed off the gorgeous table she sat at as she enjoyed a beverage. She styled her caramel tresses loosely in a straight fashion across her shoulders and opted for a high-glamour make-up palette. She put on a very leggy display in a red satin mini dress as she posed up a storm in a very lavish courtyard area.Īmanda gave herself a few extra inches in a pair of rouge strapped stilettos and simply accessorised with an array of gold bracelets. She took to her Instagram to share an array of gorgeous photographs of herself out for a luxurious evening out in the French capital. It comes after Amanda showed off her incredible figure while sharing a slew of stunning snaps on Friday. She could be seen standing with her arm around her pal, with the Heart Breakfast shielding her eyes behind a pair of dark sunglasses. Strike a pose: Earlier in the day on Saturday, Amanda shared a picture of herself posing with a friend in front of the Eiffel Tower as she wore a chic white trouser suitĮarlier in the day, Amanda shared a picture of herself posing with a friend in front of the Eiffel Tower as she wore a chic white trouser suit. |
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